QuantiFluor® dsDNA System

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

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The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.

Features - Benefits

• Specificity: Highly specific to dsDNA, minimal binding to ssDNA, RNA, protein and interfering compounds.
• Sensitivity: Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for low-concentration samples. Performs better or equal to PicoGreen® dye, and has a detection limit of 50pg/ml.
• Ease of Use: System includes all required reagents to quickly set up and quantitate dsDNA.
• Instrument Compatibility: Easy to set up on both the QuantiFluor® and GloMax®-Multi Instruments. Also can be used on the Qubit™ instrument.

Applications

• Quantification of double-stranded DNA using fluorescent methods.

Notes

Each QuantiFluor® dsDNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.

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