GSH/GSSG-Glo™ Assay can detect total glutathione, GSSG or determine GSH/GSSG ratios using fewer cells than standard methods
Limits of Detection
GSSG = 2.5nMGSH = 0.5nM
Directly measure GSSG from cells cultured in multiwell plates
| |
[GSH] nmol/106 cell |
[GSSG] nmol/106 cells |
| Cell Line |
Cells/well |
Vehicle |
Treated |
Vehicle |
Treated |
| HeLa |
5,000 |
5.18 |
4.27 |
0.08 |
1.02 |
| HepG2 |
5,000 |
2.31 |
1.41 |
0.03 |
0.40 |
| HepG2 |
10,000 |
4.57 |
3.33 |
0.05 |
0.79 |
| HepaRG |
50,000 |
5.96 |
3.24 |
0.13 |
0.71 |
| A549 |
5,000 |
5.58 |
4.31 |
0.06 |
0.33 |
| Rat hepatocytes |
20,000 |
6.24 |
5.09 |
0.30 |
1.71 |
Using the slope generated from the standard curves, glutathione RLU values are converted to micromoles GSH and GSSG.