TnT® T7 Quick for PCR DNA

TnT® T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates. For most PCR templates, the TnT® T7 Quick for PCR DNA reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.

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TnT® T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates. For most PCR templates, the TnT® T7 Quick for PCR DNA reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.

Features - Benefits

• Convenient: Directly from PCR, no cleanup necessary.
• High Yield: Up to 5 times more expressed protein than standard translation reactions with linear templates.
• Quick: One-tube reaction.
• Complete: Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.
• Good Value: One-tube format means no leftover reagents.
• Reliable: The TnT® Systems are rigorously quality controlled to ensure the highest level of transcription/translation.

Applications

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

References

1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

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