pCAT®3 Vectors

The pCAT®3 Reporter Vectors provide a basis for the quantitative analysis of factors that may regulate mammalian gene expression. The redesigned backbone of the pCAT®3 Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors, the pCAT®3 Vectors contain a different polyadenylation site located 3´ of the gene. The redesigned backbone increases expression of the reporter gene, improves in vivo vector stability and provides greater flexibility in performing manipulations.

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The pCAT®3 Reporter Vectors provide a basis for the quantitative analysis of factors that may regulate mammalian gene expression. The redesigned backbone of the pCAT®3 Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors, the pCAT®3 Vectors contain a different polyadenylation site located 3´ of the gene. The redesigned backbone increases expression of the reporter gene, improves in vivo vector stability and provides greater flexibility in performing manipulations.

Features - Benefits

• Efficient: Optimal translation efficiency.
• Robust: Increased expression with more efficient poly(A) signal.
• Clearer Results: Reduced background CAT expression.
• Compatible: Altered multiple cloning regions make vectors compatible with the pGL3 Vectors.
• Versatile: Can produce ssDNA for sequencing and mutagenesis.

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