Promega's new Wizard® Plus SV Minipreps DNA Purification System provides highly purified plasmid DNA and offers the flexibility of using either a spin or vacuum manifold format. The system incorporates a novel alkaline protease digestion step allowing the use of endA positive bacterial strains expressing endonuclease I. In this article, we demonstrate that plasmid DNA isolated using this system is optimal for demanding applications such as automated fluorescent sequencing and coupled in vitro transcription and translation systems.
Promega Notes 59, 10.
Paula Brisco, Don Creswell, Steve Ekenberg, Judy Towne, Diana Holmes, Robin Hurst, Jacqui Mendez, Susanne Selman, John Shultz, Doug Storts and Jo Grosch