In this article, we demonstrate the use of liquid handling workstations for performing cell-based assays. The feasibility of scale-up and minimal manipulation required to perform these assays makes them candidates for implementation on a variety of automated workstations. This article specifically describes the adaptation of Promega cell-based assays to the Beckman Coulter Biomek® 2000 and FX workstations in both 96- and 384-well formats. We have automated the Caspase-Glo™ 3/7 Assay, Apo-ONE® Homogeneous Caspase-3/7 Assay, CytoTox-ONE™ Homogeneous Membrane Integrity Assay, CellTiter-Glo® Luminescent Cell Viability Assay, and CellTiter-Blue™ Cell Viability Assay.
Promega Notes 85, 25–27.
Tracy Worzella and Brad Larson