Bright-Glo™ Luciferase Assay System Technical Manual

Literature # TM052

Ultrahigh-throughput quantitation of luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous process robotic systems. The Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous process systems by providing a homogeneous assay chemistry that achieves high sensitivity and approximately half-hour signal half-life without prior sample processing. These attributes also benefit scientists using fewer samples who still require high sensitivity and ease of use.
The Bright-Glo™ Luciferase Assay System is designed for use with the following culture media containing 0–10% serum: RPMI 1640, MEM-alpha,DMEM and Ham’s F12. The signal half-life under these conditions will generally exceed 25 minutes at 22°C and is independent of enzyme concentration where the luciferase concentration is <10^–9M. Other media/sera combinations also can be used, but experimental verification of assay performance is recommended in these cases.