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Notes: The pGEM^®-3Z Vector was used in subcloning. (0614)

Notes: The pGEM^®-3Zf(+) Vector was used for routine subcloning. (0077)

Notes: RNA was isolated from purified porcine enterovirus serotype 1 and oligo-d(T) primed, double stranded cDNA synthesis was accomplished using the Universal Riboclone® cDNA Synthesis System. The dsDNA was subcloned into the pGEM®-3Z Vector using of EcoRI Adaptors. (1239)

Notes: The pGEM^®-3Z Vector was used for subcloning. (0448)

Notes: The pGEM^®-3Z Vector was used for subcloning the cDNA for the ribozyme and was the template for in vitro transcription. (0904)

Notes: Ribozymes designed to cleave TNF-α cDNA were cloned into the pGEM^®-3Z Vector before being transcribed in vitro. The transcribed ribozymes were then mixed with Transfectam^® Reagent and injected into mice intraperitoneally. Tissues and cells isolated from the mice were assayed at later time points for fluor or radiolabled ribozymes. In vitro kinetic assays were also performed with ribozymes and RNA substrates labeled with T4 Polynucleotide Kinase.  (2834)

Notes: The ProFection^® Mammalian Transfection System-CaPO₄ was used to transiently transfect 293T cells. (0521)