Promega@Home-Academy

The online seminar offers an excellent opportunity to learn more about Promega‘s NanoLuc®-based tools to explore protein:protein interactions. We will introduce the versatile platform technologies NanoBiT® and NanoBRET™. These bioluminescent technologies enable to reversibly study the kinetics of homo- and heterodimerization of proteins in live cells and thus are reflective of their highly dynamic nature.

In this online seminar you will learn more about the use of CRISPR Knock-In technology in combination with the HiBiT protein tagging system. This combination enables the construction of cellular reporter assays for a wide variety of research questions. The benefits of CRISPR & HiBiT is illustrated with examples of the following research topics:

• Protein degradation (e.g. PROTACs)
• Signal transduction
• Protein secretion
• Target cell killing
• Receptor trafficking

Assays for endpoint measurements and for real-time kinetic measurements are discussed.

This online seminar will provide an overview of factors key to the successful implementation of cell-based assays in your lab. Guidance on choosing the optimal plate type and detection mode, cell handling, assay design, and timing will be given with helpful tips and tricks to avoid common pitfalls. The various assay formats available - e.g. single- vs. multiplex and endpoint vs. live-cell kinetic analysis - their benefits and limitations will be discussed with regard to assays that determine cell viability, cytotoxicity, and apoptosis.

Bioluminescent reporters have proven to be an indispensable tool for studying cellular signaling pathways and the various levels of gene expression. Beside the widely applied luciferases, i.e. firefly and renilla, new experimental reporters have been developed. In this online seminar we will guide you through the key steps of a gene reporter experiment along with helpful tips and tricks. Furthermore, the benefits of the next-generation reporter enzyme NanoLuc® luciferase will be presented and highlight be showing real application data.

This online seminar provides an overview of the newest and most sensitive detection methods for cell viability, cytotoxicity and apoptosis. Focusing on the latest developments for endpoint assays and kinetic assays where you can see changes of the cell health status in real-time. We will show how to use these assays for classical in vitro approaches as well as in 3D-cell culture models and how you can improve the value of your experimental data by multiplexing assays in the same well and combine the results to get the whole picture.

During this online seminar you will learn more about optimizing your complete RNA-workflow for reverse transcription real-time quantitative PCR (RT-qPCR) experiments. We will introduce you to the following steps of gene expression analysis: planning of the experiment, isolation of RNA from different sample types, reverse transcription as well as highly accurate qPCR quantification and data analysis. In addition we provide you with helpful tips and tricks for your daily laboratory work to make your experiments more successful.

The development of antibody-based biologics requires a cell-based functional assay that reflects the mechanism-of-action for potency evaluation, quality control and manufacturing lot release. In this online seminar we will introduce both, state-of-the-art and innovative bioluminescent reporter bioassays for Fc-receptor mediated cellular response (ADCC and ADCP) and our newest T Cell Activation, Growth Factor & Cytokine as well as Immune Checkpoint Bioassays.

Immunological detection of analytes is often performed using heterogeneous methods such as Western blotting and ELISA. Both are labor-intensive multistep procedures that often do require overnight incubation. In this online seminar we introduce a sensitive, luminescence-based, and homogenous alternative. You will learn how Lumit™ Immunoassay can simplify your workflow and yield faster results. These benefits will be emphasized by real-lab data for detection of cytokines, post-translational modifications, and antibody binding. Furthermore, we will illustrate how you can easily build your own Lumit™ Immunoassay.

HaloTag^® is a powerful self-labeling protein tag designed to covalently bind one of many synthetic HaloTag^® ligands. Upon expression as a HaloTag^® fusion protein, various features can be easily appended to a protein of interest (POI) to facilitate the investigation of its function. In this online seminar, we will have a closer look at some of the numerous applications of the HaloTag^® technology, including:

• protein purification
• analysis of protein interactions, e.g. protein:protein, protein:ligand, and protein:DNA
• cellular imaging / super-resolution microscopy
• pulse-chase analysis
• receptor trafficking
• targeted protein degradation

Cellular metabolism comprises a dynamic network of pathways that undergo reprogramming in response to external and internal signals. Metabolite levels provide valuable information about pathway activity, and plate-based coupled-enzyme reaction assays can provide a rapid method for measuring specific metabolites. In this online seminar you will learn more about Promega’s easy-to-use assays to measure metabolic activity, including glucose uptake, lactate, glutamine, lipids, oxidative stress and dinucleotide detection assays. These non-radioactive, bioluminescent methods are powerful research tools for studying cell health.

Learn more about new assays and tools developed from the NanoLuc^® Luciferase. This small and signal-intense luciferase served as starting point for the development of versatile protein reporter systems – the so called NanoBiT^® and HiBiT Protein Tagging System. The NanoBiT^® System allows the development of assays suitable for monitoring the dynamics of protein:protein interactions and the fast and homogenous quantification of proteins in ELISA-like immunoassays. The HiBiT Protein Tagging System enables the quantification of cellular changes in protein concentration, even at low-endogenous expression levels, and is therefore used mainly for research on signal transduction and protein degradation.

This online seminar will focus on a new generation of small molecules that hold significant therapeutic potential by inducing degradation of target proteins. Characterizing and optimizing protein degraders (e.g. PROTACs, IMiDs, and molecular glues) for degradation efficacy represents a significant challenge. Currently, the availability of live cell assays to interrogate the multiple steps required for targeted protein degradation is severely lacking. Here, we present a live-cell, NanoLuc^® luciferase-based technology platform that enables characterization of degrader compound mechanism of action using either endpoint or real-time kinetic assay formats.