BglI

Cut Site:

GCCN   NNN^▼NGGC
CGGN_▲NNN   NCCG

Notes:

1. BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer D. 37°C.

Source: Bacillus globigii.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: