Promega Customized Reporter and Fusion Vectors on Addgene
Promega created a robust and growing collection of prebuilt plasmids designed to accelerate experimental design and execution. Our luciferase reporter vectors enable sensitive, quantitative analysis of gene expression. Plasmids incorporating innovative tags such as NanoLuc®, HaloTag®, SmBiT, and LgBiT—used in advanced applications like live-cell protein-protein interaction (PPI) studies via NanoBRET™ and NanoBiT®, and target engagement assays—are now available from Addgene.
Through our partnership with Addgene, these widely used tools are available to both nonprofit and industry researchers without the need for a material transfer agreement (MTA). As a mission-driven organization, Addgene enhances scientific collaboration by offering expert guidance, educational resources and streamlined material transfer—all designed to simplify logistics and accelerate research.
Not sure which assay is right for you? Learn more about these technologies below:
Each tab below highlights a different collection of Promega vectors. You’ll find luciferase reporter vectors for gene expression studies, protein fusion vectors with versatile tagging options, and recommended NanoBiT® and NanoBRET® protein-protein interaction pairs. Search the collections below for your vector of interest and click the corresponding link to purchase directly from Addgene.
Luciferase Reporter Vectors
Promega's luciferase reporter vectors enable highly sensitive and quantitative measurement of gene expression in mammalian cells. These vectors offer flexibility with multiple luciferase options, facilitating optimized experimental design for your specific application.
Search list for your response element or reporter of interest (e.g., NFAT, Firefly). Don’t see what you’re looking for? Check out our DIY options or custom services.
| Pathway | Response Element/Promoter | Reporter | Addgene Plasmid ID | Request Details |
|---|---|---|---|---|
| Hedgehog | Gli Response Element (Gli) | Firefly | 236803 | |
| Notch | RBK-Jk Response Element | Firefly | 236804 | |
| PI3K/Akt/MAPK | Myc/Max Response Element | NanoLuc | 236805 | |
| TGFβ | SMAD3/SMAD4 Binding Element (SBE) | NanoLuc | 236807 | |
| TGFβ/BMP | Human DNA-Binding Protein Inhibitor ID-1 Promoter | Firefly | 236808 | |
| T Cell Activation IL-1 | Human IL-2 Promoter | Firefly | 236930 | |
| Ras/MEK1 | Serum Response Element (SRE) | NanoLuc | 236810 | |
| MAPK/JNK | AP1 Response Element (AP1 RE) | NanoLuc | 236811 | |
| JAK/STAT1/IFNγ | IFNγ Activation Site (GAS) Response Element | Firefly | 236813 | |
| Myc | Myc Response Element (Myc) | Firefly | 236815 | |
| ER Stress | ATF4 Response Element (ATF4 ERSE) | Firefly | 236816 | |
| Hypoxia | Hypoxia Response Element | NanoLuc | 236817 | |
| Oxidative Stress | Antioxidant Response Element | NanoLuc | 236818 | |
| JAK/STAT5 IL-3 | STAT5 Response Element | NanoLuc | 236819 | |
| STAT3 | Human G-CSF | Firefly | 236823 | |
| cAMP/PKA | Cyclic AMP Response Element (CRE) | NanoLuc | 236829 | |
| JAK/STAT1/IFNγ | IFNγ Activation Site (GAS) Response Element | NanoLuc | 236921 | |
| RhoA (Ga12/13) | Serum Response Factor Response Element | NanoLuc | 236835 | |
| DNA Damage | p53 Response Element (p53 RE) | NanoLuc | 236920 | |
| T Cell Activation IL-1 | Human IL-2 Promoter | NanoLuc | 236918 |
Protein Fusion Vectors
Promega’s protein fusion vectors encode cutting-edge tags, including NanoLuc®, HaloTag®, SmBiT, and LgBiT, enabling precise detection and tracking of proteins. These vectors facilitate advanced applications such as live-cell protein-protein interaction studies (NanoBRET® and NanoBiT®) and accurate measurement of cellular target engagement.
Search list for your protein or tag of interest (e.g., KRAS, NanoLuc). Don’t see what you’re looking for? Check out our DIY options or custom services.
| Protein | Tag | Terminus | Addgene Plasmid ID | Request Details |
|---|---|---|---|---|
| NRF2 | LgBiT | C | 237127 | |
| NRF2 | SmBiT | C | 237126 | |
| NRF2 | LgBiT | N | 237125 | |
| NRF2 | SmBiT | N | 237124 | |
| MAFG | LgBiT | N | 237123 | |
| MAFG | SmBiT | N | 237122 | |
| MAFG | LgBiT | C | 237121 | |
| MAFG | SmBiT | N | 237120 | |
| CALM1 | LgBiT | N | 237119 | |
| CALM1/ADC1.1 | LgBiT-SmBiT | Bidirectional | 237118 | |
| AR | LgBiT | N | 237117 | |
| CALM1/ADC1.2 | LgBiT-SmBiT | Bidirectional | 237116 | |
| ADCY8 | SmBiT | N | 237115 | |
| CALM1/ADCY8 | LgBiT-SmBiT | Bidirectional | 237114 | |
| AR | SmBiT | C | 237113 | |
| AR/AR | LgBiT-SmBiT | Bidirectional | 237155 | |
| BCL6 | LgBiT | N | 237112 | |
| BCL6 | SmBiT | N | 237111 | |
| BCL6 | LgBiT | C | 237110 | |
| BCL6 | SmBiT | C | 237109 | |
| NCOR2 | LgBiT | N | 237108 | |
| NCOR2 | SmBiT | N | 237107 | |
| NCOR2 | LgBiT | C | 237106 | |
| NCOR2 | SmBiT | C | 237105 | |
| HER1 | LgBiT | C | 237104 | |
| HER3 | SmBiT | C | 237103 | |
| P2RX7 | SmBiT | C | 237102 | |
| P2RX7 | LgBiT | C | 237101 | |
| CRAF | SmBiT | C | 237100 | |
| PIK3R1 | LgBiT | C | 237098 | |
| PIK3R1 | SmBiT | C | 237097 | |
| PIK3R1 | LgBiT | N | 237096 | |
| PIK3R1 | SmBiT | N | 237095 | |
| PIK3CA | LgBiT | C | 237094 | |
| PIK3CA | SmBiT | C | 237093 | |
| PIK3CA | LgBiT | N | 237092 | |
| PIK3CA | SmBiT | N | 237091 | |
| TUBEs | LgBiT | C | 237088 | |
| TUBEs | LgBiT | N | 237087 | |
| CYBC1 | LgBiT | N | 237086 | |
| CYBB | S | C | 237085 | |
| CYBC1/CYBB | LgBiT-SmBiT | Bidirectional | 237084 | |
| ZNRF3 | SmBiT | C | 237083 | |
| RNF43 | LgBiT | C | 237082 | |
| RNF43 | SmBiT | C | 237081 | |
| ZNRF3 | LgBiT | C | 237080 | |
| KRAS 4B (G12C) | SmBiT | N | 237079 | |
| KRAS 4B (G12C) | LgBiT | N | 237078 | |
| CRAF (RBD-CRD) | SmBiT | C | 237077 | |
| KRAS 4B (G12V) | LgBiT | N | 237076 | |
| BCL9 | SmBiT | C | 237075 | |
| BCL9 | SmBiT | N | 237074 | |
| BCL9 | LgBiT | C | 237073 | |
| BCL9 | LgBiT | N | 237072 | |
| Beta catenin | SmBiT | C | 237071 | |
| Beta catenin | SmBiT | N | 237070 | |
| Beta catenin | LgBiT | C | 237069 | |
| Beta catenin | LgBiT | N | 237068 | |
| KRAS (G12V)/CRAF (RBD) | LgBiT-SmBiT | Bidirectional | 237067 | |
| KRAS (WT)/CRAF (RBD) | LgBiT-SmBiT | Bidirectional | 237066 | |
| PDE4C3 | LgBiT | N | 237064 | |
| NRAS | SmBiT | N | 237063 | |
| NRAS | LgBiT | N | 237062 | |
| NRAS (Q61R) | SmBiT | N | 237061 | |
| NRAS (Q61R) | LgBiT | N | 237060 | |
| CYBA/CYBB | LgBiT-SmBiT | Bidirectional | 237059 | |
| VPS4A | SmBiT | N | 237058 | |
| VPS4A | SmBiT | C | 237057 | |
| VPS4B | SmBiT | N | 237056 | |
| VPS4B | SmBiT | C | 237055 | |
| CHMP4B | LgBiT | N | 237054 | |
| CHMP4B | LgBiT | C | 237053 | |
| PPP1CA | SmBiT | N | 237052 | |
| PPP1CA | SmBiT | C | 237051 | |
| PPP1CA | LgBiT | C | 237049 | |
| SELENOP | SmBiT-HaloTag | C | 237048 | |
| KRAS (G12D) | SmBiT | N | 237047 | |
| H3.3 | SmBiT | N | 237046 | |
| H4 | SmBiT | N | 237045 | |
| b4GALT1 | LgBiT | N | 237044 | |
| b4GALT1 | SmBiT | N | 237043 | |
| SLC35A2 | SmBiT | N | 237042 | |
| SLC35A2 | SmBiT | C | 237041 | |
| SLC35A2 | LgBiT | N | 237040 | |
| SLC35A2 | LgBiT | C | 237039 | |
| CHRM1 | LgBiT | C | 236795 | |
| CHRM1 | SmBiT | C | 236796 | |
| MDM2 | SmBiT | N | 237037 | |
| p53 | LgBiT | N | 237036 | |
| cMyc | LgBiT | C | 237035 | |
| Max | SmBiT | C | 237034 | |
| KRAS (G12D) | LgBiT | N | 237033 | |
| CRAF RBD | SmBiT | N | 237032 | |
| KRAS 4B (G12D) | SmBiT | N | 236798 | |
| CRAF (FL) | LgBiT | N | 236799 | |
| GLP-1R | SmBiT | C | 236845 | |
| ERN1 | SmBiT | N | 237030 | |
| GR | SmBiT | C | 237159 | |
| GR | LgBiT | N | 237160 | |
| CD3E | LgBiT | C | 237029 | |
| CD3E | SmBiT | C | 237028 | |
| NCK1 | LgBiT | C | 237027 | |
| NCK1 | SmBiT | C | 237026 | |
| NCK1 | LgBiT | N | 237025 | |
| NCK1 | SmBiT | N | 237024 | |
| YAP1 | NanoLuc | C | 237023 | |
| GR | HaloTag | N | 237153 | |
| GR | NanoLuc | C | 237154 | |
| PD-L1 | HaloTag | C | 237128 | |
| PD-L1 | NanoLuc | C | 237129 | |
| MAVS/IFIH1 | NanoLuc-HaloTag | Bidirectional | 237022 | |
| E6 | HaloTag | C | 237133 | |
| E6AP | NanoLuc | C | 237134 | |
| MLL1 | NanoLuc | N | 237135 | |
| Menin | HaloTag | N | 237136 | |
| BCL9L | HaloTag | C | 237137 | |
| beta-Catenin | NanoLuc | N | 237138 | |
| EED | NanoLuc | C | 237130 | |
| PRMT5 | NanoLuc | C | 237131 | |
| TAF1 | NanoLuc | C | 237132 | |
| ACT1 | NanoLuc | N | 237018 | |
| ACT1 | NanoLuc | C | 237017 | |
| PD-1/SHP2 | NanoLuc-HaloTag | Bidirectional | 237016 | |
| TCF4 | NanoLuc | N | 237015 | |
| beta-Catenin | HaloTag | C | 237014 | |
| Histone H4 | HaloTag | C | 237011 | |
| MLL1 | NanoLuc | C | 237139 | |
| WDR5 | HaloTag | C | 237140 | |
| BCL9L | HaloTag | N | 237010 | |
| NSD3 | NanoLuc | C | 237008 | |
| WDR5 | HaloTag | N | 237141 | |
| MYC | NanoLuc | C | 237142 | |
| VCP | HaloTag | N | 237006 | |
| VCP | HaloTag | C | 237005 | |
| TEAD4 | HaloTag | C | 237004 | |
| YAP1/TEAD4 | NanoLuc-HaloTag | Bidirectional | 237003 | |
| PRMT5 | NanoLuc | N | 237002 | |
| CSNK1A1 | NanoLuc | C | 237143 | |
| E-cadherin | HaloTag | C | 237144 | |
| APC | HaloTag | N | 237145 | |
| MAFG | HaloTag | N | 237001 | |
| WRN | NanoLuc | N | 237000 | |
| WRN | NanoLuc | C | 236999 | |
| BCL9 | NanoLuc | C | 237146 | |
| beta-Catenin | HaloTag | N | 237147 | |
| FBXO42 | HaloTag | C | 236998 | |
| FBXO42 | HaloTag | N | 236997 | |
| FBXO42 | NanoLuc | C | 236996 | |
| FBXO42 | NanoLuc | N | 236995 | |
| CCDC6 | HaloTag | C | 236994 | |
| CCDC6 | HaloTag | N | 236993 | |
| CCDC6 | NanoLuc | C | 236992 | |
| CCDC6 | NanoLuc | N | 236991 | |
| beta-Catenin (S45F) | HaloTag | C | 236990 | |
| TREM2 | HaloTag | C | 237148 | |
| IRF5 | HaloTag | C | 237149 | |
| IRF5 | NanoLuc | C | 237150 | |
| CKS1B | HaloTag | N | 237151 | |
| Skp2 | NanoLuc | C | 237152 | |
| TEAD2 | NanoLuc | C | 236987 | |
| TEAD3 | NanoLuc | C | 236986 | |
| MDM4 | NanoLuc | N | 236985 | |
| FKBP-12 | NanoLuc | C | 236984 | |
| FKBP-12 | NanoLuc | N | 236983 | |
| PPP3CA | HaloTag | C | 236982 | |
| PPP3CA | HaloTag | N | 236981 | |
| hVPS4A | NanoLuc | N | 236980 | |
| hVPS4A | NanoLuc | C | 236979 | |
| hVPS4A | HaloTag | N | 236978 | |
| hVPS4A | HaloTag | C | 236977 | |
| hVPS4B | HaloTag | N | 236976 | |
| hVPS4B | HaloTag | C | 236975 | |
| hCHMP4B | NanoLuc | N | 236974 | |
| hCHMP4B | NanoLuc | C | 236973 | |
| hCHMP4B | HaloTag | N | 236972 | |
| hCHMP4B | HaloTag | C | 236971 | |
| hVTA1 | NanoLuc | N | 236970 | |
| hVTA1 | NanoLuc | C | 236969 | |
| hVTA1 | HaloTag | N | 236968 | |
| hVTA1 | HaloTag | C | 236967 | |
| FBXO22 | HaloTag | C | 236966 | |
| MBP | HaloTag | N | 236965 | |
| MBP | HaloTag | C | 236964 | |
| MBP | NanoLuc | N | 236963 | |
| MBP | NanoLuc | C | 236962 | |
| FYN | HaloTag | N | 236961 | |
| FYN | HaloTag | C | 236960 | |
| FYN | NanoLuc | N | 236959 | |
| VDR | NanoLuc | C | 236958 | |
| VDR | NanoLuc | N | 236957 | |
| E-cadherin1 | NanoLuc | C | 236956 | |
| TTC7B | HaloTag | N | 236955 | |
| TTC7B | HaloTag | C | 236954 | |
| TTC7B | NanoLuc | N | 236953 | |
| TTC7B | NanoLuc | C | 236952 | |
| PP1Cg | HalTag | N | 236951 | |
| PP1Cg | HaloTag | C | 236950 | |
| PP1Cg | NanoLuc | N | 236949 | |
| PP1Cg | NanoLuc | C | 236948 | |
| SHOC2 | NanoLuc | N | 236947 | |
| CBP (bd1) | NanoLuc | C | 236945 | |
| FANCD2 (WT) | NanoLuc | N | 236944 | |
| Ubiquitin | HaloTag | N | 236943 | |
| TEAD1 | HaloTag | C | 236942 | |
| TEAD2 | HaloTag | C | 236941 | |
| TEAD3 | HaloTag | C | 236940 | |
| YAP | NanoLuc | N | 236938 | |
| TAZ | NanoLuc | N | 236937 | |
| KSR1 | HaloTag | C | 236794 | |
| KSR2 | HaloTag | C | 236793 | |
| TP53 (Y220C) | TP53(Y220C) | C | 236802 | |
| IRF5 | HaloTag | C | 236791 | |
| IRF5 | NanoLuc | C | 236792 | |
| MALT1 | NanoLuc | N | 236936 | |
| MALT1 | NanoLuc | C | 236935 | |
| EZH2 | NanoLuc | C | 236934 | |
| Suz12 | HaloTag | N | 236933 | |
| ER | HaloTag | C | 236932 | |
| ER | NanoLuc | C | 236931 | |
| BRAF (RBD) | HaloTag | C | 236879 | |
| KRAS (WT) | NanoLuc | N | 236878 | |
| ARAF | HaloTag | C | 236877 | |
| ARAF | NanoLuc | C | 236876 | |
| NRAS (WT) | NanoLuc | N | 236875 | |
| CRAF (FL) | HaloTag | C | 236874 | |
| CRAF (RBD-CRD) | HaloTag | C | 236873 | |
| CRAF (CRD-RBD)/KRAS (G12C) | NanoLuc-HaloTag | Bidirectional | 236872 | |
| KRAS | HaloTag | N | 236871 | |
| CRAF (FL) | NanoLuc | C | 236870 | |
| KRAS (G12C)/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236869 | |
| KRAS (G12C)/CRAF (FL) | NanoLuc-HaloTag | Bidirectional | 236868 | |
| KRAS (G12D)/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236867 | |
| HRAS (WT) | nan | nan | 236866 | |
| KRAS (WT)/CRAF (RBD-CRD) | NanoLuc-HaloTag | Bidirectional | 236865 | |
| NRAS/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236864 | |
| NRAS (Q61R) | NanoLuc | N | 236863 | |
| NRAS (Q61R)/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236862 | |
| HRAS (WT)/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236861 | |
| NRAS (Q61K)/CRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236860 | |
| KRAS (G12V) | NanoLuc | N | 236859 | |
| KRAS (G12C) | HaloTag | N | 236858 | |
| KRAS (G12D) | HaloTag | N | 236857 | |
| KRAS (G13D) | HaloTag | N | 236856 | |
| PIK3CA | NanoLuc | N | 236855 | |
| NRAS (Q61K) | NanoLuc | N | 236854 | |
| KRAS (2B WT)/BRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236853 | |
| NRAS (Q61R)/BRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236852 | |
| NRAS (WT)/BRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236851 | |
| HRAS (WT)/BRAF (RBD) | NanoLuc-HaloTag | Bidirectional | 236850 | |
| SOS1 | NanoLuc | N | 236848 | |
| XIAP | NanoLuc | N | 237212 | |
| UHMK1 | NanoLuc | N | 237211 | |
| DGKb | NanoLuc | C | 237163 | |
| DGKb | NanoLuc | N | 237186 | |
| DGKb | NanoLuc | C | 237167 | |
| KDR | NanoLuc | C | 237210 | |
| TOM7 | NanoLuc | N | 237166 | |
| TOM20 | NanoLuc | C | 237179 | |
| TOM20 | NanoLuc | N | 237174 | |
| PNCK | NanoLuc | C | 237209 | |
| HSD17B13 | NanoLuc | N | 237180 | |
| HSD17B13 | NanoLuc | C | 237170 | |
| IRAK2 | NanoLuc | N | 237181 | |
| EGR2 | NanoLuc | N | 237171 | |
| PTPN2 | NanoLuc | N | 237182 | |
| PGDH | NanoLuc | C | 237184 | |
| PGDH | NanoLuc | N | 237177 | |
| EIF2AK3 | NanoLuc | C | 237169 | |
| EIF2AK3 | NanoLuc | N | 237188 | |
| KSR1 | NanoLuc | C | 237175 | |
| AKT3 | NanoLuc | C | 237208 | |
| BRD9 (BD) | NanoLuc | N | 237205 | |
| BRPF1 (BD) | NanoLuc | N | 237204 | |
| CECR2 (BD) | NanoLuc | N | 237203 | |
| TAF1 | NanoLuc | N | 237165 | |
| TAF1 | NanoLuc | N | 237201 | |
| CA2 | NanoLuc | N | 237187 | |
| CA2 | NanoLuc | C | 237164 | |
| EIF2AK2 (252-551) | NanoLuc | C | 237172 | |
| EGFR (L858R T790M) | NanoLuc | C | 237199 | |
| SRPK1 | NanoLuc | C | 237178 | |
| MDM2 | NanoLuc | N | 237198 | |
| PI4KB | NanoLuc | C | 237197 | |
| HSP90AB1 | NanoLuc | N | 237196 | |
| cIAP1 | NanoLuc | N | 237195 | |
| DCLK1 (Iso1) | NanoLuc | C | 237193 | |
| CIT | NanoLuc | N | 237191 | |
| TGFBR1 | NanoLuc | C | 237190 | |
| NLRP3 | NanoLuc | C | 237189 | |
| DSTYK | NanoLuc | N | 237162 | |
| TP53 (Y220C) | HiBiT | N | 236914 | |
| GCGR | secHiBiT | N | 236837 | |
| TP53 | HiBiT | C | 236838 | |
| SRPK1 | HiBiT | N | 236908 | |
| WRN | HiBiT | N | 236906 | |
| WRN | HiBiT | C | 236905 | |
| CLK3 | HiBiT | N | 236904 | |
| CLK3 | HiBiT | C | 236903 | |
| IFIH1 | HiBiT | C | 236902 | |
| IFIH1 | HiBiT | N | 236901 | |
| Beclin-1 | HiBiT | N | 236900 | |
| Beclin-1 | HiBiT | C | 236899 | |
| CHRM1 | HiBiT | N | 236898 | |
| SHOC2 | HiBiT | N | 236897 | |
| UBASH3A | HiBiT | C | 236896 | |
| ADAR | HiBiT | C | 236895 | |
| VPS4A | HiBiT | N | 236894 | |
| VPS4B | HiBiT | N | 236893 | |
| ADARB1 | HiBiT | C | 236892 | |
| TDP1 | HiBiT | N | 236891 | |
| CFTR (WT) | HiBiT | C | 236890 | |
| BRPF1 | NanoLuc | C | 236889 | |
| KDR | secHiBiT | N | 236888 | |
| SLC35A2 | HiBiT | N | 236887 | |
| SLC35A2 | HiBiT | C | 236886 | |
| TP53 | HiBiT | N | 236885 | |
| IRAK4 | HiBiT | N | 236884 | |
| HRH2 | HiBiT | N | 236883 | |
| HSD17B13 | HiBiT | N | 236839 | |
| HSD17B13 | HiBiT | C | 236840 |
NanoBiT® Protein-Protein Interaction Pairs
Promega’s recommended NanoBiT® protein-protein interaction (PPI) pairs combine SmBiT- and LgBiT-protein fusion vectors for ready-to-use live-cell PPI assays. NanoBiT® PPI assays offer simple, low-background detection of interaction association and/or dissociation in real time.
Search the table to see if we have a recommended orientation for your protein-protein interactions. Is your protein pair of interest not listed here? Check our full list of protein fusion vectors, DIY options, or custom TRS services.
| SmBiT Protein | SmBiT Terminus | SmBiT Plasmid Number & Link | LgBiT Protein | LgBiT Terminus | LgBiT Plasmid Number & Link | Bidirectional Vector Number & Link | Request Details |
|---|---|---|---|---|---|---|---|
| Max | C | 237034 | cMyc | C | 237035 | N/A | |
| MDM2 | N | 237037 | p53 | N | 237036 | N/A | |
| AR | C | N/A | AR | N | N/A | 237155 | |
| GR | C | 237159 | GR | N | 237160 | N/A | |
| AVPR2 | C | 236800 | ARRB2 | N | 236801 | N/A | |
| CRAF RBD | N | 237032 | KRAS(G12D) | N | 237033 | N/A | |
| CRAF(RBD-CRD) | C | 237077 | KRAS(G12D) | N | 237033 | N/A | |
| CRAF RBD | N | N/A | KRAS 4B(G12V) | N | N/A | 237067 | |
| CRAF RBD | N | N/A | KRAS (WT) | N | N/A | 237066 | |
| CRAF | C | N/A | BRAF | C | N/A | CS1679D24 | |
| CRAF | C | N/A | CRAF | C | N/A | 238808 | |
| BRAF | C | N/A | BRAF | C | N/A | 238809 | |
| PRKACA | N | N/A | PRKAR2A | N | N/A | 237156 | |
| EGFR(1-785) | C | N/A | EGFR(1-785) | C | N/A | 238756 | |
| cMET(1-960) | C | N/A | cMET(1-960) | C | N/A | 238767 | |
| ARRB2 | N | CS1603B399 | β2AR | C | NB1221 | N/A | |
| V2R | C | 236800 | ARRB2 | N | CS1603B399 | N/A |
NanoBRET® Protein-Protein Interaction Pairs
Promega’s recommended NanoBRET® protein-protein interaction (PPI) pairs feature NanoLuc®- and HaloTag®-protein fusion vectors to enable sensitive, real-time measurement of protein-protein interactions in live cells.
Search the table to see if we have a recommended orientation for your protein-protein interactions. Is your protein pair of interest not listed here? Check our full list of protein fusion vectors, DIY options, or custom TRS services.
| NanoLuc Protein | NanoLuc Terminus | NanoLuc Vector Number & Link | HaloTag Protein | HaloTag Terminus | HaloTag Vector Number & Link | Bidirectional Number & Link | Request Details |
|---|---|---|---|---|---|---|---|
| KRasG12C | N | N/A | cRAF CRD-RBD | C | N/A | 236872 | |
| bRAF | C | N/A | KRasG12C | N | N/A | 238566 | |
| KRasG12C | N | N/A | cRAF RBD | C | N/A | 236869 | |
| KRasG12C | N | N/A | cRAF(FL) | C | N/A | 236868 | |
| KRasG12C | N | 238582 | bRAF RBD | C | 236879 | N/A | |
| ARAF | C | 236876 | KRasG12C | N | 236858 | N/A | |
| PI3KCA1 | N | NV3901 | KRasG12C | N | 236858 | N/A | |
| SOS1 cat | N | 236848 | KRasG12C | N | 236858 | N/A | |
| bRAF | C | N/A | KRasG12D | N | N/A | 238563 | |
| KRasG12D | N | N/A | cRAF (FL) | C | N/A | 238579 | |
| KRasG12D | N | N/A | cRAF RBD | C | N/A | 236867 | |
| KRasG12D | N | N/A | cRAF CRD-RBD | C | N/A | 238576 | |
| ARAF | C | 236876 | KRasG12D | N | 236857 | N/A | |
| PI3KCA1 | N | NV3901 | KRasG12D | N | 236857 | N/A | |
| SOS1 cat | N | 236848 | KRasG12D | N | 236857 | N/A | |
| bRAF | C | N/A | KRasG12V | N | N/A | 238565 | |
| KRasG12V | N | N/A | cRAF RBD | C | N/A | 238572 | |
| KRasG12V | N | N/A | cRAF (FL) | C | N/A | 238571 | |
| ARAF | C | 236876 | KRasG12V | N | 238570 | N/A | |
| KRasWT | N | N/A | cRAF CRD-RBD | C | N/A | 236865 | |
| KRasWT | N | 236878 | cRAF (FL) | C | 236874 | N/A | |
| KRas4A | N | N/A | cRAF RBD | C | N/A | 236849 | |
| bRAF RBD | C | N/A | HRasWT | N | N/A | 236850 | |
| HRasWT | N | 236866 | cRAF (FL) | C | 236874 | N/A | |
| HRasWT | N | N/A | cRAF RBD | C | N/A | 236861 | |
| bRAF RBD | C | N/A | NRasWT | N | N/A | 236851 | |
| NRasWT | N | 236875 | cRAF (FL) | C | 236874 | N/A | |
| NRasQ61R | N | N/A | cRAF RBD | C | N/A | 236862 | |
| bRAF RBD | C | N/A | NRasQ61R | N | N/A | 236852 | |
| NRasQ61R | N | 236875 | cRAF (FL) | C | 236874 | N/A | |
| NRasQ61K | N | N/A | cRAF RBD | C | N/A | 236860 | |
| NRasQ61K | N | 236854 | cRAF (FL) | C | 236874 | N/A | |
| ARAF | C | 236876 | ARAF | C | 236877 | N/A | |
| bRAF | C | 238569 | bRAF | C | 238568 | N/A | |
| cRAF | C | 238828 | cRAF | C | 236874 | N/A | |
| ARAF | C | 236876 | bRAF | C | 238568 | N/A | |
| ARAF | C | 236876 | cRAF (FL) | C | 236874 | N/A | |
| bRAF | C | N/A | cRAF | C | N/A | Link to catalog | |
| IFIH1 | N | N/A | MAVS | N | N/A | 237022 | |
| EZH2 | N/A | 236934 | Suz12 | N/A | 236933 | N/A | |
| ER | N/A | 236931 | ER | N/A | 236932 | N/A | |
| GR | N/A | 237153 | GR | N/A | 237154 | N/A | |
| SHP2 | N | N/A | PD-1 | C | N/A | 237016 | |
| TCF4(biased) | N | N/A | beta-Catenin | C | N/A | 238680 | |
| YAP1(biased) | N | N/A | TEAD4 | C | N/A | 238679 | |
| YAP1 | N | N/A | TEAD4 | C | N/A | 237003 | |
| YAP1 | N | N/A | TEAD4 | C | N/A | 238696 | |
| YAP1 | N | N/A | TEAD2 | C | N/A | 238669 |