PureYield™ Plasmid Miniprep System
Up to 15μg of Transfection-Ready Plasmid from 3ml Cultures
- Rapid, 10-minute miniprep protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
Catalog Number:
Size
Catalog Number: A1223
Catalog Number: A1222
Plasmid Minipreps in Minutes
The PureYield™ Plasmid Miniprep System isolates up to 15μg of transfection-quality plasmid DNA from 600μl to 3ml of bacterial culture in 10 minutes. The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the performance in applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The PureYield™ Plasmid Miniprep System can be used with centrifugation or vacuum-based protocols, and includes a convenient lysis/neutralization indicator dye.
This miniprep kit can be used to isolate any plasmid from E. coli hosts but works most efficiently when the plasmid is up to 20,000bp in size. For best results, use plasmids that are 10,000bp or less. Purified plasmid can be used without further manipulation for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques. When used for in vitro transcription reactions, the isolated plasmid DNA should be supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Cell Lysis Buffer (CLC) |
A145D | 1 × 11ml |
|
Neutralization Solution (NSC) |
A157D | 1 × 36ml |
|
Endotoxin Removal Wash (ERB) |
A158D | 1 × 20.5ml |
|
Column Wash Solution (CWC) |
A159D | 1 × 9ml |
|
Elution Buffer (EBB) |
A160C | 1 × 10ml |
|
PureYield™ Minicolumns |
A166B | 2 × 50 columns |
|
PureYield™ Collection Tubes |
A167B | 2 × 50 tubes |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Lysis/neutralization indicator licensed under pending patents assigned to Zymo Research Corporation.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Cell Lysis Buffer (CLC) |
A145C | 1 × 27ml |
|
Neutralization Solution (NSC) |
A157C | 1 × 90ml |
|
Endotoxin Removal Wash (ERB) |
A158C | 2 × 27ml |
|
Column Wash Solution (CWC) |
A159C | 1 × 25ml |
|
Elution Buffer (EBB) |
A160C | 1 × 10ml |
|
PureYield™ Minicolumns |
A166B | 5 × 50 columns |
|
PureYield™ Collection Tubes |
A167B | 5 × 50 tubes |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Lysis/neutralization indicator licensed under pending patents assigned to Zymo Research Corporation.
Related Products
Similar Products
PureYield™ Plasmid Miniprep Start-Up Kit
Bundle of 750 minipreps, vacuum manifold and free vacuum pump.
A6812
PureYield™ Plasmid Midiprep System
Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.
A2492, A2495, A2496
PureYield™ Plasmid Maxiprep System
1mg of transfection-ready plasmid from 250ml cultures in 1 hour.
A2392, A2393
Frequently Used With
Molecular Biology Lab Guide
A resource designed for scientists just embarking on their career, focusing on fundamental technologies and techniques.
GEN1978
Vac-Man® Jr. Laboratory Vacuum Manifold
For rapid, effective nucleic acid purification. Process two samples at a time.
A7660, A3110
FuGENE® 6 Transfection Reagent
Proven, dependable performance. Best for everyday use with common cell lines.
E2691, E2692, E2693
HB101 Competent Cells
E. coli strain ready for transformation. No blue/white selection.
L2015, L2011
JM109 Competent Cells
E. coli strain ready for transformation; offers blue/white selection.
L2005, L1001, L2001
ViaFect™ Transfection Reagent
High-efficiency, low-toxicity transfection of many cell types, including iPS cells.
E4981, E4982
Vac-Man® Laboratory Vacuum Manifold
For fast, effective nucleic acid purification. Process up to 20 samples simultaneously.
A7231
