Flexi® Rabbit Reticulocyte Lysate System
Optimized Translation for Greater Protein Production
- Increase protein production as much as fourfold over standard lysate reactions
- For easy optimization the endogenous magnesium concentration is provided for each lot of Flexi® Lysate
- The Flexi® System contains three Amino Acid Mixtures, providing a choice of radioisotopes
Catalog Number:
Size
Catalog Number: L4540
Translation Optimization for Mg2+ and K+ Concentrations
The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice to add or omit DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.
The Flexi® Rabbit Reticulocyte Lysate  System, when optimized, can increase the quantity of protein produced as much as fourfold over a standard lysate reaction. The Flexi® System contains three Amino Acid Mixtures, enabling different choices of radioisotopes. Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control (only full-length luciferase is active).
For more information, see the Protocols & Applications Guide.
Applications:
- Optimizing translation of low-expressing messages.
- Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
- Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
- Investigation of translational control.
- Screening of chemical libraries for effect on translation.
Reference:
- Beckler, G.S. et al. (1995) Methods in Molecular Biology 37, 215–32.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Amino Acid Mixture Minus Cysteine |
L447C | 1 × 50μl | 1mM | |
|
Flexi® Rabbit Reticulocyte Lysate |
L455A | 5 × 200μl | ||
|
Luciferase Control RNA |
L456A | 1 × 10μl | View Product | |
|
Luciferase Assay Wells |
L457A | 1 × 3 each | ||
|
Magnesium Acetate |
L458A | 1 × 100μl | 25mM | |
|
Potassium Chloride |
L459A | 1 × 200μl | 2.5M | |
|
Luciferase Assay Reagent |
L486A | 1 × 250μl | ||
|
Amino Acid Mixture Minus Leucine |
L995A | 1 × 50μl | 1mM | |
|
Amino Acid Mixture Minus Methionine |
L996A | 1 × 50μl | 1mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Technical Bulletin for detailed storage information.
Related Products
Similar Products
Rabbit Reticulocyte Lysate, Nuclease-Treated
Identify mRNA species, characterize proteins, investigate transcription/translation control.
L4960
RiboMAXâ„¢ Large-Scale RNA Production Systems
Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.
P1280, P1300
Transcendâ„¢ Non-Radioactive Translation Detection System
Non-radioactive detection of proteins synthesized in vitro.
L5070
FluoroTectâ„¢ GreenLys in vitro Translation Labeling System
Fluorescent labeling and detection of in vitro synthesized proteins.
L5001
Frequently Used With
Ribo m7G Cap Analog
Enhances translation efficiency in many reticulocyte-based reactions.
P1711, P1712
RNasin® Ribonuclease Inhibitor
The most-trusted product for broad-spectrum inhibition of common eukaryotic RNases.
N2111, N2115, N2511, N2515, N2611, N2615
Luciferase Control RNA
Unique functional control for in vitro translation reactions. Uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail producing functional luciferase when translated. Control reactions monitored by a luciferase assay.
L4561
