HaloTEV Protease
Cleave and Recover Target Protein in a Single Step
- Easy to covalently remove HaloTEV from your purified protein with HaloLinkâ„¢ Resin
- Proteins can be purified tag-free in a single step as the HaloLink™ Resin will bind both HaloTag® protein tag and the HaloTEV protease
- Supplied at a concentration of 5u/μl
Catalog Number:
Size
Catalog Number: G6601
Catalog Number: G6602
HaloTag®-Protease Fusion for Rapid Target Protein Recovery
HaloTEV Protease is a fusion between the HaloTag® protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)). HaloTEV Protease enables covalent immobilization on HaloLink™ Resin and removal from a cleavage reaction in a single-step purification. The covalent capture of HaloTEV Protease improves purity of the final target protein and assures the improved recovery of the TEV protease.
It's easy to remove HaloTEV from your purified protein with HaloLinkâ„¢ Resin.
References
- Ryu, M.-J. et al. (2012) Oncogenic KRas expression in postmitotic neurons leads to S100A8-S100A9 protein overexpression and gliosis. J. Biol. Chem. 287, 22948–58.
- Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr. Purif. 76, 154–64.
Coomassie®-stained gel of HaloTEV Protease showing 1 unit (U) of protein with the specific activity of 1 unit/µg.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
HaloTEV Protease |
G660A | 1 × 200μl | 5u/μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
HaloTEV Protease |
G660B | 1 × 800μl | 5u/μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources found
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