Assays for Biochemical Targets
This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest. Find assays to measure the activity of a broad range of enzymes, from kinases and phosphatases to HDACs, hydroxylases and many more! Simply type in your enzyme of interest to find the correct assay.
Search for assay
| Enzyme Name | Gene Symbol | Keywords | Assay |
|---|---|---|---|
| Glycosyltransferase | |||
| Sugar chain synthetic enzyme, GalNAc | |||
| Polypeptide GalNAc Transferase 1 | GALNT1 | Glycosylation, neurological disease | UDP-Glo™ Assay |
| Polypeptide GalNAc Transferase 4 | GALNT4 | Glycosylation, neurological disease | UDP-Glo™ Assay |
| Sugar chain synthetic enzyme, GlcNAc | |||
| Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase 3 | MGAT3 | Cell surface antigen | UDP-Glo™ Assay |
| Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase 5 | MGAT5 | Cell surface antigen | UDP-Glo™ Assay |
| B 1,3-N-acetylgucosaminyltransferase 2 | B3GNT2 | Cell surface antigen | UDP-Glo™ Assay |
| 0-linked N-acetylglucosaminyltransferase | OGT | Glycosylation, neurological disease | UDP-Glo™ Assay |
| Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 | POMGNT1 | Glycosylation, neurological disease | UDP-Glo™Assay |
| Sugar chain synthetic enzyme, galactose | |||
| B-1,4 Galactosyltransferase 1 | B4GalT1 | Glycosylation, neurological disease | UDP-Glo™ Assay |
| B-1,4-Galactosyltransferase 2 | B4GalT2 | Glycosylation, neurological disease | UDP-Glo™ Assay |
| Sugar chain synthetic enzyme, sialic acid | |||
| ST3 Beta-Gal alpha -2,3-Sialyltransferase 1 | ST3GAL1 | Cell surface antigen, cancer | UMP/CMP-Glo™ |
| ST6 Gal Sialyltransferase 1 | ST6GAL1 | Cell surface antigen, cancer | UMP/CMP-Glo™ |
| Sugar chain synthetic enzyme, fucose | |||
| Fucosyltransferase 2 | FUT2 | Cell surface antigen, cancer | GDP-Glo™ Assay |
| Fucosyltransferase 3 | FUT3 | Cell surface antigen, cancer | GDP-Glo™ Assay |
| Fucosyltransferase 5 | FUT5 | Cell surface antigen, cancer | GDP-Glo™ Assay |
| Fucosyltransferase 7 | FUT7 | Cell surface antigen, cancer | GDP-Glo™ Assay |
| Fucosyltransferase 8 | FUT8 | Cell surface antigen, cancer | GDP-Glo™ Assay |
| Sugar chain synthetic enzyme, mannose | |||
| Mannosyltransferase | _ | _ | GDP-Glo™ Assay |
| Glycosylation enzyme | |||
| Blood Group B Transferase | GTB | Blood type, cell surface antigen | UDP-Glo™ Assay |
| Bacteria pathogenic toxins | |||
| Clostridium difficile Toxin B | Toxin B (TcdB) | _ | UDP-Glo™ Assay |
| Ubiquitin-proteasome | |||
| SUMO-adding enzyme | |||
| SUMO-activating enzyme subunit 1 / ubiquitin-like modifier activating enzyme 2 Heterodimer | SAE1 / UBA2 | Protein stabilization, transcription factor activity control | AMP-Glo™ Assay |
| SUMO conjugating enzyme | UbcH9 | Protein stabilization, transcription factor activity control | AMP-Glo™ Assay |
| NEDD additional enzyme | |||
| Ubiquitin-like modifier activating enzyme 3 | UBA3 | Protein stabilization, transcription factor activity control | AMP-Glo™ Assay |
| NEDD8 activating enzyme E1 subunit 1 | NAE1 (APPBP1) | Protein stabilization, transcription factor activity control | AMP-Glo™ Assay |
| Ubiquitin ligase | |||
| Ubiquitin Ligase E1 | UBA1 | Proteolysis / quality control | AMP-Glo™ Assay |
| Ubiquitin Ligase E2 | UBE2D2 / UbcH5b | Proteolysis / quality control | AMP-Glo™ Assay |
| Ubiquitin Ligase E3 | CARP2 | Proteolysis / quality control | AMP-Glo™ Assay |
| Proteasome | |||
|
Proteasome Chymotrypsin-Like, Trypsin-Like, Caspase-Like |
PSMA / PSMB / PSMC / PSMD / PSME / PSMF / PSMG | Protein degradation, protein quality control, ubiquitin disease, neurodegeneration, cancer | Proteasome-Glo™, Cell-Based System |
|
Proteasome Chymotrypsin-Like, Trypsin-Like, Caspase-Like |
PSMA / PSMB / PSMC / PSMD / PSME / PSMF / PSMG | Protein degradation, protein quality control, ubiquitin disease, neurodegeneration, cancer | Proteasome-Glo™ Assay |
| Protein modification | |||
| Deadenylation | |||
| Poly A-Deadenylases | PARN | mRNA stability, gene expression control | AMP-Glo™ Assay |
| Protein poly ADP- ribosylation | |||
| Poly ADP-Ribose transferases 1 | PARP1 | DNA damage repair, cancer | AMP-Glo™ Assay |
| chaperone | |||
| Heat Shock proteins | HSPs | Protein synthesis, quality control | ADP-Glo™ Assay |
| Epigenetics | |||
| Methylase | |||
| DNA Methyltransferase | DNMT1, 3a | Epigenetics, cancer | MTase-Glo™ Assay |
| Histone Methyltransferase | EZH1, 2 | Epigenetics, cancer | MTase-Glo™ Assay |
| EHMT1, 2 | _ | Epigenetics, cancer | MTase-Glo™ Assay |
| SET7, 9 | _ | Epigenetics, cancer | MTase-Glo™ Assay |
| PRMT5 | _ | Epigenetics, cancer | MTase-Glo™ Assay |
| DOT1L | _ | Epigenetics, cancer | MTase-Glo™ Assay |
| Histone demethylating enzyme | |||
| JumonjiC histone demethylases | JMJD2A | Cancer, glycolipid metabolism and metabolism-related gene expression | Succinate-Glo™ Assay |
| JumonjiC histone demethylases | JMJD2C | – | Succinate-Glo™ Assay |
| JumonjiC histone demethylases | JARID1A | – | Succinate-Glo™ Assay |
| JumonjiC histone demethylases | UTX | – | Succinate-Glo™ Assay |
| Histone deacetylase | |||
| HDAC1-11 | HDAC1-11 | Epigenetics, cancer, gene expression control | HDAC-Glo™ I / II Assay |
| HDAC4, 5, 7, 9 | HDAC4, 5, 7, 9 | Epigenetics, cancer, gene expression control | HDAC-Glo™ Class IIa Assay |
| HDAC2 | HDAC2 | Epigenetics, cancer, gene expression control | HDAC-Glo™ 2 Assay |
| Sirtuin deacetylase | |||
| SIRT1-6 | SIRT1-6 | Anti-aging, energy metabolism, | SIRT-Glo™ Assay |
| Nucleic acid synthesis and degradation enzyme | |||
| Nucleotidase, AMP-degrading enzyme | |||
| 5'-nucleotidase (5'-NT) or Ecto-5'-nucleotidase | CD73 (NT5E) | Liver function markers, gastrointestinal disease | AMP-Glo™ Assay |
| Ligase | |||
| DNA Ligases | _ | Antibiotics, antibacterial agents | AMP-Glo™ Assay |
| Nuclease | |||
| Ribonucleases | RNase | _ | AMP-Glo™ Assay |
| Helicase | |||
| Helicases | _ | Transcriptional regulation, DNA synthesis | ADP-Glo™ Assay |
| Signaling | |||
| cAMP-degrading enzyme | |||
| Phosphodiesterase 3B | PDE3B | cAMP signaling | AMP-Glo™ Assay |
| Phosphodiesterase 4B | PDE4B | cAMP signaling | AMP-Glo™ Assay |
| Phosphodiesterase 8A | PDE8A | cAMP signaling | AMP-Glo™ Assay |
| Phosphodiesterase 10A | PDE10A | cAMP signaling | AMP-Glo™ Assay |
| Phosphodiesterase 11A | PDE11A | cAMP signaling | AMP-Glo™ Assay |
| Phosphodiesterase 3B | PDE3B | cAMP signaling | cAMP-Glo™ Assay |
| Phosphodiesterase 4B | PDE4B | cAMP signaling | cAMP-Glo™ Assay |
| Phosphodiesterase 8A | PDE8A | cAMP signaling | cAMP-Glo™ Assay |
| Phosphodiesterase 10A | PDE10A | cAMP signaling | cAMP-Glo™ Assay |
| Phosphodiesterase 11A | PDE11A | cAMP signaling | cAMP-Glo™ Assay |
| Phosphodiesterase 3B | PDE3B | cAMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 4B | PDE4B | cAMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 8A | PDE8A | cAMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 10A | PDE10A | cAMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 11A | PDE11A | cAMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Intracellular cAMP level change | |||
| Non-enzymatic assay (cAMP measurement) | - | GPCR signal analysis | GloSensor™ cAMP Assay |
| cGMP-degrading enzyme | |||
| Phosphodiesterase 5 | PDE5 | cGMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 6 | PDE6 | cGMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Phosphodiesterase 9 | PDE9 | cGMP signaling | PDE-Glo™ Phosphodiesterase Assay |
| Intracellular cGMP level change | |||
| Non-enzymatic assay (cGMP measurement) | - | GPCR signal analysis | GloSensor™ cGMP Assay |
| Low molecular weight GTP-ase | |||
| Ras | Ras | Cancer | GTPase-Glo™ Assay |
| RhoA | RhoA | Cancer, cardiovascular disease, cell shape change | GTPase-Glo™ Assay |
| RAB | Rab5A | Neurological disorders, immune disorders, membrane traffic | GTPase-Glo™ Assay |
| Trimeric G protein | |||
| Gi alpha subunit | GNAI1 | cAMP signaling | GTPase-Glo™ Assay |
| GTase activating protein (GAP) | |||
| Neurofibromin 1 | NF1 | Cancer | GTPase-Glo™ Assay |
| Guanine nucleotide exchange factor (GEF) | |||
| Regulator of chromosome condensation 1 | RCC1 | Cancer | GTPase-Glo™ Assay |
| GTase activating protein (GAP) | |||
| RanGAP1 | RANGAP1 | Cancer | GTPase-Glo™ Assay |
| Kinase (proteins, peptides, lipids, sugars, etc.) | |||
| Protein Kinases (All) | PKA, PKC, EGFR, FGFR and others | Signaling | ADP-Glo™Assay |
| Metabolism and biosynthesis | |||
| Succinic acid Co synthesis, aerobic metabolism | |||
| Succinate CoA Synthetase | SUCLG | TCA cycle, aerobic metabolism | Succinate-Glo™ Assay / GTPase-Glo |
| tRNA synthetase | |||
| Aminoacyl tRNA synthetases | _ | Protein synthesis, metabolism | AMP-Glo™ Assay |
| Energy metabolism enzyme / redox enzyme / other metabolic enzyme | |||
| Oxidase, Reductase, metabolic enzyme | H6PD, GLUC1, 2, LDH, G6PD, | Cancer, metabolism, diabetes | NAD (P) H-Glo™ Detection System |
| Hydrogen peroxide detection | |||
| Catalase, Ascorbate peroxidases, NAD (P) H oxidase, Peroxidase | CAT, APX, NOX, GPX | Cancer, energy metabolism, | ROS-Glo™ H2O2 Assay (H2O2 detection only) |
| Cytotoxicity (apoptosis) | |||
| caspase 3/7 | CASP3, 7 | Apoptosis | Caspase-Glo® 3/7 Assay |
| caspase 8 | CASP8 | Apoptosis | Caspase-Glo® 8 Assay |
| caspase 9 | CASP9 | Apoptosis | Caspase-Glo® 9 Assay |
| caspase 1 | CASP1 | Apoptosis, inflammation, inflammasome | Caspase-Glo® 1 Assay |
| ADME | |||
| Drug metabolism | |||
| CYP3A4 | _ | Drug metabolism, liver cell differentiation | P450-Glo™ CYP3A4 Assay with Luciferin-IPA |
| CYP3A4, CYP3A5, CYP3A7 | _ | Drug metabolism, liver cell differentiation | P450-Glo™ CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay |
| CYP3A4, CYP3A5, CYP3A7 | _ | Drug metabolism, liver cell differentiation | P450-Glo™ CYP3A4 Assay (Luciferin-PFBE) |
| CYP1A1, CYP1B1, CYP3A7 | _ | Drug metabolism, liver cell differentiation |
P450-Glo™ CYP1A1
Assay (Luciferin-CEE) P450-Glo™ CYP1B1 Assay (Luciferin-CEE) |
| CYP1A2, CYP2C8, CYP2C9, CYP2J2, CYP4A11, CYP4F3B, CYP19 | _ | Drug metabolism, liver cell differentiation |
P450-Glo™ CYP1A2
Assay (Luciferin-ME) P450-Glo™ CYP2C8 Assay (Luciferin-ME) |
| CYP1A2 | _ | Drug metabolism | P450-Glo™ CYP1A2 Induction / Inhibition Assay |
| CYP2B6 | _ | _ | P450-Glo™ CYP2B6 Assay |
| CYP2C9 | _ | Drug metabolism, liver cell differentiation | P450-Glo™ CYP2C9 Assay (Luciferin-H) |
| CYP3A4, CYP3A5, CYP3A7, CYP4F12 | _ | Drug metabolism, liver cell differentiation |
P450-Glo™ CYP3A4
Assay (Luciferin-BE) P450-Glo™ CYP3A7 Assay (Luciferin-BE) |
| CYP2C19, CYP1A1, CYP1A2 | _ | Drug metabolism | P450-Glo™ CYP2C19 Assay |
| CYP2D6, CYP1A1, CYP1A2, CYP2B6 | _ | Drug metabolism | P450-Glo™ CYP2D6 Assay |
| CYP3A7 | _ | Drug metabolism | Luciferin-3A7 |
| CYP4A11 | _ | Drug metabolism | Luciferin-4A |
| CYP4F2, CYP4F3 | _ | Drug metabolism | Luciferin-4F2 / 3 |
| CYP4F12 | _ | Drug metabolism | Luciferin-4F12 |
| CYP2J2, CYP4F12 | _ | Drug metabolism | Luciferin-2J2 / 4F12 (ester) |
|
High activity: CYP1A1, CYP1A2, CYP1B1, CYP2D6 moderate: CYP2B6, CYP2C9, CYP2C19, CYP2J2, CYP3A4, CYP3A5, CYP4A11, CYP4F2, CYP4F3A, CYP4F3B, CYP4F12, CYP19 |
_ | Drug metabolism | Luciferin-MultiCYP (ester) |
| Drug transporter | |||
| P-glycoprotein 1 | Pgp1 / MDR1 | P-glycoprotein 1 | Pgp-Glo™ Assay System with P-glycoprotein |
| ABC transporters | Pgp1 / MDR1, MDR2, MDR3 | Drug metabolism | ADP-Glo™ Assay |
| Monoamine oxidase | |||
| Monoamine oxidase | MAOA, MAOB | Drug metabolism, Phase I enzyme | MAO-Glo™ Assay System (by cell species) |
| N-acetylated | |||
| NAT2 | NAT2 | Drug metabolism, Phase II enzymes | Luciferin-NAT2 |