Finding the Right Protein Purification System

Gary Kobs
Promega Corporation
Publication Date: 2004

Abstract

Researchers often need to purify a single protein for further detailed study. One method for isolating a target protein involves affinity purification. A recombinant fusion protein that is "tagged" with a stretch of amino acids or other small protein is expressed in bacteria. The fusion protein is purified based on specific biochemical properties that are conferred by the tag. Here we discuss factors that influence the choice of protein tag and purification system.

Cell Notes 9, 2–5.